감염근관에서 Black-Pigmented Bacteria의 동정에 대한 연구
DETECTION OF BLACK-PIGMENTED BACTERIA IN INFECTED ROOT CANALS
권은경, 김은숙, 곽주석, 이황, 이수종, 임미경,
소속 상세정보
권은경 ( kwon Eun-Kyoung ) - 원광대학교 치과대학 보존학교실
김은숙 ( Kim Eun-Sook ) - 원광대학교 치과대학 치과보존학교실
곽주석 ( Kwak Ju-Seog ) - 원광대학교 치과대학 치과보존학교실
이황 ( Lee Hwang ) - 원광대학교 치과대학 치과보존학교실
이수종 ( Lee Su-Jong ) - 원광대학교 치과대학 치과보존학교실
임미경 ( Im Mi-Kyung ) - 원광대학교 치과대학 치과보존학교실
KMID : 0362320020270010054
Abstract
Black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonass endodontalis, Porphyromonas gingicalis, Prevotella intermedia, and Prevotella nigresceus. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections.
The aim of this study was to identify P.endodontalis, P.gingivalis, P.intermedia, and P.nigrescens by using special potency disk test, filter paper spot test, 16S rRNA gene-directed PCR, and API 32A.
Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and/or apical periodontitis. Conventional laboratory methods were used for identification of the strains of black pigmented bacteria. Eighteen of 33 samples were positive for the growth of black-pigmented bacteria. Five colonies were cultured from each pure cultured colonies from Brucella agar plate. Seventy seven colonies were positive for the growth of black-pigmented bacteria.
Thirty three of 77(42.6%) were identifed as P.nigrescens, 10 of 77(12.9%) were P.gingivalis, 6 of 77(7.8%) were P.endodontalis, 10 of 77(12.9%) were P.intermedia. On the contraty the reference strains of P.nigrescens, experimental strains of P.nigrescens was sensitive to kanamycin in special potency disk test.
16S rRNA gene PCR and API test after rapid presumptative identification methods. such as special potency disk test and filter paper spot test, would be accurate detection methods for black-pigmented bacteria.
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